节杆菌启动子活性片段的克隆与分析任务书

 2021-09-30 22:50:21

1. 毕业设计(论文)的内容和要求

论文内容 启动子是基因表达调控的重要顺式元件, 也是基因工程表达载体的一个重要组成部分。

启动子的克隆对于构建基因工程载体, 表达目的蛋白有着重要的意义。

但是关于节杆菌能够识别的启动子的报道比较少,本文的研究内容主要是构建能够在Arthrobacterium CGMCC3842中复制的两种节杆菌启动子探针质粒,其中pAR-Cm以氯霉素为筛选标记,pAR-GFP以绿色荧光蛋白为筛选标记,为后期在节杆菌中寻找启动子打下基础。

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2. 参考文献

[1]Koch,C.,Schumann,P. and Stackebrandt,E.(1995).Reclassification of Micrococcus agilis(Ali-Cohen 1889) to the Genus Arthrobacter as Arthrobacter agilis comb.nov.and Emendation of the Genus Arthrobacter.Int.J.Syst.Bacteriol 45,837-839.[2]Hagedorn,C. and Holt,J.G.(1975).A nutritional and taxonomic survey of Arthrobacter soil isolates.Can.J.Microbiol.21,353-361.[3] Skyring,G.W. and Quadling,C.(1969).Soil bacteria: comparisons of rhizosphere and nonrhizosphere populations,Can.J.Microbiol.15,473-488.[4]Lowe,W.E. and Gray,T.R.G.(1972).Ecological studies on coccoid bacteria in a pine forest soil-I.Classification.Soil Biology and Biochemistry 4,459-467.[5]Soumare,S.and Blondeau,R.(1972).[Microbiological properties of soils in Northern France:Arthrobacter extent].Ann.Inst.Pasteur(Pairs)123,239-249.[6] Jones,D.and Keddie,R.(2006) The Genus Arthrobacter.In The prokaryotes (Dworkin,M.,Falkow,S.,Rosenberg,E.,Schleifer,K.-Hand Stackebrandt,E.,eds),pp.945-960.Springer New York.[7]Fong, N.J.,Burgess, M.L.,Barrow,K.D. and Glenn, D.R.(2001). Carotenoid accumulation in the psychrotrophic bacterium Arthrobacter agilis in response to thermal and salt stress. Appl.Microbiol.Biotechnol.56,750-756.[8]Margesin,R.and Schinner,F.(1996).Bcaterial heavy metal-tolerance-extreme resistance to nickel in Arthrobacter spp.strains.J.Basic Microbiol.36,269-282.[9]Nordin,K.,Unell,M. and Jansson,J.K.(2005).Novel 4-chlorophenol degradation gene cluster and degradation route via hydroxyquinol in Arthrobacter chlorophenolicus A6.Appl.Environ.Microbiol.71.6538-6544[10]Strong,L.C., Rosendahl, C.,Johnson,G.,Sadowsky, M.J. and Wackell, L.P.(2002).Arthrobacter aurescens TC1 metabolizes diverse s-triazine ring compounds.Appl. Environ. Microbiol.68,5973-5980.[11]Fredrickson, J.K. et al.(2004). Geomicrobiology of high-level nuclear waste-contaminated vadose sediments at the hanford site,washington state.Appl.Environ.Microbiol.70,4230-4241.[12]Mingodin,E.F.etal.(2006).Secrets of soil survival revealed by the genome sequence of Arthrobacter aurescens TC1,Plos Genet.2,20214-20220.[13]Bernasconi,E.,Valsangiaconmo,C.,Peduzzi,R.,Carota,A.,Moccetti,T.and Funke,G.(2004).Arthrobacter wodluwensis subacute infective endocarditis: case report and review of the literature.Clin.infect.Dis.38,e27-31.[ 14 ]Schmitt-John T , Engels W. Promoter constructions for efficient secretion expression in Streptomyces lividans[J ] . Appl. Microbiol.Biotechnol ,1992 ,36 :493~498.[ 15]Rachael LN , Robert WW, Raymond LR. Eukaryotic DNA fragments which actpromoters for a plasmidgene[J ] . Nature ,1979 ,277 :324~325.[ 16]Donna MW, Elizabeth JD , Paul SL. Cloning restriction fragments that promote expression of a gene inBacillus subtilis [J ] . J . Bacterol ,1981 ,146 :1162~1165.[ 17]Mark MZ , Dairena FG, Denis S , et al. Chromogenic identification of genetic regulatory signals in Bacillussubtilis based on expression of cloned Pseudomonas gene [J ] . Proc. Natl. Acad. Sci. USA ,1993 ,80 : 1101~1105.[ 18]李奉锡,李育阳. 在枯草杆菌中有启动子功能的噬菌体T5DNA 片断的克隆[J ] . 遗传学报,1987 ,14 (6) :468~474.[ 19]Scopione RC , De Camargo SS , Schenberg AC , et al. A new promoter2probe vector for Saccharomtc scerevisiae using fungal glucoamylase cDNA as the reporter gene[J ] . Yeast , 1993 ,9 :599~605.[ 20]Sidhu RC , Mathewes S , Bollon AP. Selection of secretory protein2encoding genes by fusion in Saccharomyces erevisiae [J ] . Gene ,1991 ,107 :111~118.[ 21 ]Fordor I , Kranikova OV , Berets E , et al. Cloning structure and features of a S accharomyces cerevisiae DNA ragment causing the expression of reporter genes[J ] . Mol. Biol ,1990 ,24 :1411~1418.[ 22 ]Luo XM. Cloning and characterization of three Aspergillus niger promoters[J ] . Gene ,1995 , 163 :127~131.[23 ] 金红,王以光. 麦迪霉素产生菌具有启动功能的DNA 片段的克隆和分析[J ] . 微生物学报,1994 ,34 (6) :415~421

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