1. 毕业设计（论文）的内容和要求
论文内容：利多卡因是一种局部麻醉药且可用于治疗室性心动过速，为可卡因的一种衍生物。
由于目前利多卡因的合成多采用化学法，存在反应条件剧烈、有机溶剂污染等缺陷，而生物法具有突出的环境友好性，反应温和，区域选择性和立体选择性高等优势，但目前还未有生物法合成利多卡因的相关报道。
本论文旨在筛选出能够高效催化合成利多卡因的菌株，同时通过基因工程手段找到能够产生催化反应的目标酶，为利多卡因生物合成提供支撑。
2. 参考文献
1.Shichao Z, Ji L, Sampson A, et al. Amide Synthesis via Aminolysis of Ester or Acid with an Intracellular Lipase[J]. ACS Catal. 2018, 8, 88568865. 2.Mark R, Petchey, Gideon Grogan. Enzyme‐Catalysed Synthesis of Secondary and Tertiary Amides[J]. Adv. Synth. Catal. 10.1002/adsc.201900694.3.S. van Pelt, R. L. M. Teeuwen, M. H. A. Janssen, et al. Pseudomonas stutzeri lipase: a useful biocatalyst for aminolysis reactions[J]. Green Chem., 2011, 13, 1791.4.Janaina Marques Almeida, Robson Carlos Alnoch, Emanuel Maltempi Souza, et al. Metagenomics: Is it a powerful tool to obtain lipases for application in biocatalysis?[J]. BBA - Proteins and Proteomics,2020,1868(2).5.任红威,连少翰,王雪,张优,张金锋,段二红.低共熔溶剂取代危化品的应用进展[J].过程工程学报,2018,18(S1):1-13.6.姜恬,冯旭东,李岩,李春.底物特异性的生物催化与酶设计改造[J].化工进展,2019,38(01):606-614.7.Gotor-Fernandez, V.; Gotor, V. Enzymatic aminolysis and ammonolysis processes in the preparation of chiral nitrogenated compounds[J]. Curr. Org. Chem. 2006, 10, 11251143.8.Nechab, M. Azzi, N. Vanthuyne, N. et al. Highly selective enzymatic kinetic resolution of primary amines at 80 C: A Comparative Study Of Carboxylic Acids and Their Ethyl Esters as Acyl Donors[J]. Org. Chem. 2007, 72, 69186923.9.Tufvesson, P. Annerling, A. Hatti-Kaul, R. et al. Solvent-free Enzymatic Synthesis of Fatty Alkanolamides[J]. Biotechnol. Bioeng. 2007, 97, 447453(28). Pitzer, J. Steiner, K. Amides in Nature and Biocatalysis.[J]. Biotechnol. 2016, 235, 3246.
