1. 1. 毕业设计（论文）的内容、要求、设计方案、规划等
内容：
1、对分离得到的番泻叶苷A进行水解,获得其苷元。
2、研究番泻叶苷A及其苷元在肌肉坏死组织中的分布。
2. 参考文献（不低于12篇）
［1］江苏医学院，中药大辞典(下册)[M].上海:上海人民出版社，1977:2409-2409［2］林秀珍, 郭世铎. 大承气汤,芍甘汤,大黄素和番泻甙对肠梗阻大鼠肠粘膜组胺水平的影响［J］. 中国中药杂志，1992, 17(7):42-429
［3］顾波，金琦. 番泻叶的药理作用及临床应用［J］. 首都医药，2003,10(22)：45-46［4］钟济秀, 李唯佳. 大断下片治疗大鼠脾虚泄泻的实验研究［J］. 浙江中医杂志，1992, 27(6):274-275［5］杨文修，田在善. 番泻总甙对豚鼠结肠带平滑肌细胞电活动的影响［J］. 中国药理学报，1993, 14(5):430-432［6］程红科. 番泻叶的临床应用及其不良反应［J］. 安徽中医临床杂志，2002, 14(4):229-230［7］兰梅，王新，刘娜，等. 番泻叶提取物对豚鼠结肠平滑肌细胞的收缩作用［J］. 第四军医大学学报，2002, 23(4):289-291
［8］Kaneshiro T, Morioka T, Inamine M, et al. Anthraquinone derivative emodin inhibits tumor- associated angiogenesis through inhibition of extracellular signal-regulated kinase 1/2 phos- phorylation[J]. Eur J Pharmacol, 2006,553(1-3):46-47［9］Kwak HJ, Park MJ, Park CM, et al. Emodin inhibits vascular endothelial growth factor-A- induced angiogenesis by blocking receptor-2 (KDR/Flk-1) phosphorylation[J]. Int J Cancer, 2006,118(11): 2711-2712 ［10］Huang Q, Shen HM, Ong CN. Emodin inhibits tumor cell migration through suppression of the phosphatidylinositol 3-kinase-Cdc42/Rac1 pathway[J]. Cell Mol Life Sci,2005,62(10):1167-1169［11］Huang Q, Shen HM, Ong CN. Inhibitory effect of emodin on tumor invasion through suppression of activator protein-1 and nuclear factor-kappaB[J]. Biochem Pharmacol, 2004,68 (2):361-363［12］Olsen BB, Bjorling-Poulsen M, Guerra B. Emodin negatively affects the phosphoinositide 3-kinase/AKT signalling pathway: a study on its mechanism of action[J]. Int J Biochem Cell Biol, 2007,39(1):227-230［13］Brown M, Bellon M, Nicot C. Emodin and DHA potently increase arsenic trioxide interferon alpha induced cell death of HTLV-I transformed cells via generation of reactive oxygen species and inhibition of Akt and AP1[J]. Blood, 2007,109(4):1653-1654［14］Yang J, Li H, Chen YY, et al. Anthraquinones sensitize tumor cells to arsenic cytotoxicity in vitro and in vivo via reactive oxygen species-mediated dual regulation of apoptosis[J]. Free Radic Biol Med, 2004,37(12):2027-2028［15］Su YT, Chang HL, Shyue SK, et al. Emodin induces apoptosis in human lung adenocarcinoma cells through a reactive oxygen species-dependent mitochondrial signaling pathway[J]. Biochem Pharmacol, 2005,70(2):229-230
