1. 毕业设计（论文）的内容和要求
1.文献查阅学会利用计算机在中国期刊网、维普数据网、Springerlink、CA、SCI、Elsevier等等网络检索资源上检索、查阅与产酯酶海洋菌的筛选与鉴定的分析相关的文献，掌握文献查阅的方法。
2.文献阅读及综述仔细阅读与所做课题相关的国内外文献，了解相关的背景知识以及目前国内外的研究动态，会系统分析，撰写文献综述。
3.明确实验任务，拟定实验方案根据所查文献的内容，结合大学所学课程相关知识，确定实验内容及方案，拟定科学可行的研究计划。
2. 参考文献
[1] ADHIKARY R, MANDAL V. l -theanine: A potential multifaceted natural bioactive amide as health supplement [J]. Asian Pacific Journal of Tropical Biomedicine, 2017, 7(9): 842-8.[2] JAMWAL S, SINGH S, GILL J S, et al. L-theanine prevent quinolinic acid induced motor deficit and striatal neurotoxicity: Reduction in oxido-nitrosative stress and restoration of striatal neurotransmitters level [J]. European Journal of Pharmacology, 2017, 811: 171-9.[3] YONEDA Y. An l -Glutamine Transporter Isoform for Neurogenesis Facilitated by l -Theanine [J]. Neurochemical Research, 2017, 42(10): 2686-97.[4] ROGERS P J, SMITH J E, HEATHERLEY S V, et al. Time for tea: mood, blood pressure and cognitive performance effects of caffeine and theanine administered alone and together [J]. Psychopharmacology, 2008, 195(4): 569-77.[5] LIU J, SUN Y, ZHANG H, et al. Theanine from tea and its semi-synthetic derivative TBrC suppress human cervical cancer growth and migration by inhibiting EGFR/Met-Akt/NF-κB signaling [J]. European Journal of Pharmacology, 2016, 791(297-307.[6] HIDESE S, OTA M, WAKABAYASHI C, et al. Effects of chronic l-theanine administration in patients with major depressive disorder: an open-label study [J]. Acta Neuropsychiatrica, 2016, 29(2): 72-9.[7] LEHOTAY J. Isolation of L-theanine from Plant Material using a Molecularly Imprinted Polymer [J]. Journal of Liquid Chromatography umami component of tea, from glutamine and ethylamine with bacterial γ-glutamyltranspeptidase [J]. Enzyme Microbial Technology, 2002, 31(6): 884-9.[19] SHUAI Y Y, TAO Z, BO J, et al. Development of efficient enzymatic production of theanine by γ-glutamyltranspeptidase from a newly isolated strain of Bacillus subtilis, SK11.004 [J]. J Sci Food Agric, 2010, 90(15): 2563-7.[20] BINDAL S, GUPTA R. L-theanine synthesis using gamma-glutamyl transpeptidase from Bacillus licheniformis ER-15 [J]. J Agric Food Chem, 2014, 62(37): 9151-9.[21] CHEN X, SU L, WU D, et al. Application of recombinant Bacillus subtilis γ-glutamyltranspeptidase to the production of l-theanine [J]. Process Biochemistry, 2014, 49(9): 1429-39.[22] 郭亮, 沈微, 王正祥等. 生物转化法生产茶氨酸的重组大肠杆菌的构建[J]. 食品与生物技术学报, 2005, 24 (2): 41-45. [23] 王贤波, 王丽鸳, 成浩等. 茶氨酸生物合成工程菌构建[J]. 茶叶科学, 2007, 27 (1): 61-66.[24] 贾晓鹤, 陈莉, 赵宁伟等. 生物转化法应用重组谷氨酰转肽酶合成L-茶氨酸[J]. 食品工业科技. 2008, 29(02): 116-119.
