蔗糖磷酸化酶的异源高效可溶性表达与纯化任务书

 2021-11-03 22:43:04

1. 毕业设计(论文)的内容和要求

1.查阅资料,整理蔗糖磷酸化酶性质、催化机理和应用。

2.了解学习大肠杆菌表达系统。

3.查询提高异源蛋白可溶性表达的方法。

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2. 参考文献

1. Wang M , Wu J , Wu D . Cloning and expression of the sucrose phosphorylase gene in Bacillus subtilis and synthesis of kojibiose using the recombinant enzyme[J]. Microbial Cell Factories, 2018, 17(1):23.2. Chen L , Sun P , Li Y , et al. A fusion protein strategy for soluble expression of Stevia glycosyltransferase UGT76G1 in Escherichia coli[J]. 3 Biotech, 2017, 7(6):356.3. Cai R , Chen C , Li Y , et al. Improved soluble bacterial expression and properties of the recombinant flavonoid glucosyltransferase UGT73G1 from, Allium cepa[J]. Journal of Biotechnology, 2017, 255:9-15.4. Goedl C , Sawangwan T , Wildberger P , et al. Sucrose phosphorylase: A powerful transglucosylation catalyst for synthesis of α-D-glucosides as industrial fine chemicals[J]. Biocatalysis and Biotransformation, 2010, 28(1):10-21.5. Desmet T , Soetaert W , Bojarov, Pavla, et al. Enzymatic Glycosylation of Small Molecules: Challenging Substrates Require Tailored Catalysts[J]. Cheminform, 2012, 18(35):1078610801.6.侯顾伟, 马江锋, 隋姗姗, et al. 蔗糖磷酸化酶制备及应用的研究进展[J]. 中国酿造, 2010, 29(6):17-20.7.张蕙. 重组蔗糖磷酸化酶在大肠杆菌BL21中的表达优化及酶学性质分析[D]. 2018.8.叶慧. 长双歧杆菌蔗糖磷酸化酶在大肠杆菌中的表达及发酵优化[D]. 2015.9.A novel sucrose phosphorylase from the metagenomes of sucrose-rich environment: isolation and characterization[J]. World Journal of Microbiology and Biotechnology, 2012, 28(9):2871-2878.10. Su L , Wang T , Yu L , et al. Highly efficient extracellular expression of naturally cytoplasmic[J]. Journal of Chemical Technology bbert. Selective expression of the soluble product fraction in Escherichia coli cultures employed in recombinant protein production processes[J]. Applied Microbiology and Biotechnology, 2010, 87(6):p.2047-2058.18. Yadong H , Yuxia Q , Xiaoshan W , et al. High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli[J]. BMC Biotechnology, 2010, 10.19.Nick S. Berrow, K. Bssow, B. Coutard,等. Recombinant protein expression and solubility screening in Escherichia coli: a comparative study [J]. Acta Crystallographica, 62(10):1218-1226.20.GoedlC,SchwarzAA,NidetzkyB.2007.RecombinantsucrosephosphorylasefromLeuconostocmesenteroides: characterization, kinetic studies of transglucosylation, and application of immobilisedenzyme for production of alpha-D-glucose 1-phosphate. Journal of Biotechnology, 129(1): 77-86. 21.范翠英,冯利兴,樊金玲,果德安,刘璇. 2012.重组蛋白表达系统的研究进展.生物技术, 22(2): 76-80. 22.MuellerM,NidetzkyB.2007.TheroleofAsp-295inthecatalyticmechanismofLeuconostoc mesenteroidessucrosephosphorylaseprobedwithsite-directedmutagenesis.FebsLetters,581(7): 1403-1408.

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