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1. 毕业设计（论文）的内容和要求
1、文献查阅 掌握文献查阅的一般方法，学会利用计算机在中国期刊网、维普数据网、超星数字图书馆、美国化学学会（ACS）、CA 、SCI等检索资源上查阅与产脲酶菌种筛选的研究相关的文献。
2、文献阅读及综述仔细阅读与所做课题相关的中英文文献，了解相关背景以及目前国内外的研究动态，撰写文献综述。
3、明确实验任务，拟定实验方案根据所查文献的内容，结合大学所学课程相关知识，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
1.实验前期的设计以及制备材料方法的查询与建立2.根据文献方法配置培养基并筛选分离3.纯化所得菌，获得纯菌4.基因组抽提，PCR，16sDNA鉴定，ncbi比对，绘制进化树
3. 参考文献
1. Altschul, S. F., T. L. Madden, A. A. Schaffer, J. Zhang, Z. Zhang, W. Miller,and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generationof protein database search programs. Nucleic Acids Res. 25:33893402.2. Anderson, S., V. D. Appanna, J. Huang, and T. Viswanatha. 1992. A novelrole for calcite in calcium homeostasis. FEBS Lett. 308:9496.3. Boon, N., J. Goris, P. De Vos, W. Verstraete, and E. M. Top. 2000. Bioaug-mentation of activated sludge by an indigenous 3-chloroaniline-degradingComamonas testosteroni strain, I2gfp. Appl. Environ. Microbiol. 66:29062913.4. Boon, N., J. Goris, P. De Vos, W. Verstraete, and E. M. Top. 2001. Geneticdiversity among 3-chloroaniline- and aniline-degrading strains of the Co-mamonadaceae. Appl. Environ. Microbiol. 67:11071115.5. Boquet, E., A. Boronat, and A. Ramos-Cormenzana. 1973. Production ofcalcite (calcium carbonate) crystals by soil bacteria is a general phenomenon.Nature (London) 246:527529.6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation ofmicrogram quantities of protein utilizing the principle of protein-dye bind-ing. Anal. Biochem. 72:248254.7. Burne, R. A., and Y. Y. Chen. 2000. Bacterial ureases in infectious diseases.Microbes Infect. 2:533542.8. Castanier, S., G. Le Me ′tayer-Levrel, and J. P. Perthuisot. 1999. Ca-carbon-ates precipitation and limestone genesisthe microbiologist point of view.Sediment. Geol. 126:923.9. Douglas, S., and T. J. Beveridge. 1998. Mineral formation by bacteria innatural microbial communities. FEMS Microbiol. Ecol. 26:7988.10. Felske, A., A. D. L. Akkermans, and W. M. De Vos. 1998. In situ detection ofan uncultured predominant Bacillus in Dutch grassland soils. Appl. Environ.Microbiol. 64:45884590.11. Felske, A., A. Wolterink, R. Van Lis, W. M. De Vos, and A. D. L. Akkermans.1999. Searching for predominant soil bacteria: 16S rDNA cloning versusstrain cultivation. FEMS Microbiol. Ecol. 30:137145.12. Ferris, F. G., and L. G. Stehmeier. January 1993. Bacteriogenic mineralplugging. U.S. patent 5,143,15513. Fujita, Y., F. G. Ferris, R. D. Lawson, F. S. Colwell, and R. W. Smith. 2000.Calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicro-biol. J. 17:305318.14. Greenberg, A. E., L. S. Clesceri, and A. D. Eaton (ed.). 1992. Standardmethods for the examination of water and wastewater, 18th ed. AmericanPublic Health Association, Washington, D.C.15. Hammes, F., N. Boon, G. Clement, J. de Villiers, S. D. Siciliano, and W.Verstraete. Molecular, biochemical and ecological characterisation of a bio-catalytic calcification reactor. Appl. Microbiol. Biotechnol., in press.16. Hammes, F., A. Seka, S. De Knijf, and W. Verstraete. 2003. A novel ap-proach to calcium removal from calcium-rich industrial wastewater. WaterRes. 37:699704.17. Hammes, F., and W. Verstraete. 2002. Key roles of pH and calcium metab-olism in microbial carbonate precipitation. Re. Environ. Sci. Bio/Technol.1:37.18. Jukes, T. H., and C. R. Cantor. 1969. Evolution of protein molecules, p.21132. In H. N. Munro (ed.), Mammalian protein metabolism. AcademicPress, Inc., New York, N.Y.19. Kawaguchi, T., and A. W. Decho. 2002. A laboratory investigation of cya-nobacterial extracellular polymeric secretions (EPS) in influencing CaCO 3polymorphism. J. Cryst. Growth 240:230235.20. Knorre, H., and W. Krumbein. 2000. Bacterial calcification, p. 2531. InR. E. Riding and S. M. Awramik (ed.), Microbial sediments. Springer-Verlag, Berlin, Germany.21. Kowalchuk, G., J. Stephen, W. De Boer, J. Prosser, T. Embley, and J.Woldendorp. 1997. Analysis of ammonia-oxidizing bacteria of the beta sub-division of the class Proteobacteria in coastal sand dunes by denaturinggradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomalDNA fragments. Appl. Environ. Microbiol. 63:14891497.22. Maidak, B. L., J. R., Cole, T. G. Lilburn, C. T. Parker, Jr., P. R. Saxman,R. J. Farris, G. M. Garrity, G. J. Olsen, T. M. Schmidt, and J. M. Tiedje.2001. The RDP-II (Ribosomal Database Project). Nucleic Acids Res. 29:173174.23. McConnaughey, T. A., and F. F. Whelan. 1997. Calcification generates pro-tons for nutrient and bicarbonate uptake. Earth Sci. Rev. 42:95117.24. McKay, D. S., E. K. Gibson, Jr., K. Thomas-Keprta, H. Vali, C. S. Romanek,S. J. Clemett, X. D. F. Chillier, C. R. Maechling, and R. N. Zare. 1996.Search for past life on Mars: possible relic biogenic activity in Martianmeteorite ALH84001. Science 273:924930.25. Mobley, H. L. T., and R. P. Hausinger. 1989. Microbial ureases: significance,regulation and molecular characterization. Microbiol. Rev. 53:85108.26. Mobley, H. L. T., M. D. Island, and R. P. Hausinger. 1995. Molecular biologyof microbial ureases. Microbiol. Rev. 59:451480.
4. 毕业设计（论文）计划
起讫日期 设计（论文）各阶段工作内容 备 注2021.1.1-2021.1.11 查阅文献，熟悉课题，写开题报告2021.2.16-2021.4.1 熟悉实验相关技能操作 2021.4.2-2021.5.31 实验的系统进行 2021.6.1-2021.6.15 整理实验数据，总结实验结果，论文写作
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
