全文总字数：1748字
1. 毕业设计（论文）的内容和要求
本论文拟从半夏根际土壤中分离、筛选对半夏软腐病有拮抗作用的根际拮抗细菌，并对高效拮抗菌进行鉴定，旨在寻找对半夏软腐生物防治有效的拮抗菌株，以期为促进半夏的无公害生产提供技术支持，从而提高半夏药材的产量和质量。
2. 实验内容和要求
实验室已经获得了半夏软腐病的病原菌以及半夏催芽、育苗等基本技术方法，学生需要在后期执行课题过程中熟练进行微生物学实验的操作，例如培养基制备、无菌操作、微生物培养等。
3. 参考文献
Yao, H., Jiao, X., and Wu, F. 2006. Effects of continuous cucumber cropping and alternative rotations under protected cultivation on soil microbial community diversity. Plant Soil, 284: 195203. doi:10.1007/s11104-006-0023-2.Yin, C., Hulbert, S.H., Schroeder, K.L., Mavrodi, O., Mavrodi, D., Dhingra, A., et al. 2013. Role of bacterial communities in the natural suppression of Rhizoctonia solani bare patch disease of wheat (Triticum aestivum L.). Appl. Environ. Microbiol. 79: 74287438. doi:10.1128/AEM.01610-13. PMID:24056471.Yin, F.J., Qu, X.Y., Zeng, W., and Shu, S. 2009. Study on the autointoxication of aquatic extracts from different parts of Coptis chinensis. J. Chin. Med. Mater. 32: 329330. [In Chinese.]Zeyaullah, M., Kamli, M.R., Islam, B., Atif, M., Benkhayal, F.A., Nehal, M., et al. 2009. Metagenomics: an advanced approach for non-cultivable micro-organisms. Biotechnol. Mol. Biol. Rev. 4: 049054.Zhang, C.L., Sun, Q., and Ye, Q. 2005. Obstacle effect of continuous cropping on Salvia miltiorrhiza growth. Acta Bot. BorealiOccident. Sin. 25: 10291034. [In Chinese.]Zhang, X.H. 2010. Occurrence and control measures of Pinellia ternata root-rot disease. Xiandai Hortic. 2: 002. [In Chinese.]Zhang, X.L., Pan, Z.G., Zhou, X.F., and Ni, W.Z. 2007. Autotoxicity and continuous cropping obstacles. Chin. J. Soil Sci. 4: 033. [In Chinese].
4. 毕业设计（论文）计划
2021.02-2021.03 半夏软腐病拮抗菌筛选；2021.03-2021.04 筛选出的半夏软腐病拮抗菌的鉴定；2021.04-2021.05 半夏软腐病拮抗菌拮抗物质的初步鉴定。
