小球藻生物合成叶黄素的代谢调控任务书

 2022-01-30 07:01

全文总字数:2015字

1. 毕业设计(论文)的内容和要求

叶黄素对提高人体视力及免疫力具有重要作用的抗氧化剂,可以缓解强化辐射等。

微藻可以高产叶黄素,将研究小球藻产叶黄素的代谢调控机制。

2. 实验内容和要求

微藻培养;叶黄素的HPLC分析;通过强氧化剂及植物生长调节剂等调控微藻叶黄素含量。

3. 参考文献

1.Ambati, R. R., Gogisetty, D., Aswathanarayana, R. G., Ravi, S., Bikkina, P. N., Lei, B. and Su, Y. (2019) Industrial potential of carotenoid pigments from microalgae: Current trends and future prospects. Critical Reviews in Food Science and Nutrition, 59, 1880-1902.2.Barbosa, M. J., Zijffers, J. W., Nisworo, A., Vaes, W., van Schoonhoven, J. and Wijffels, R. H. (2005) Optimization of biomass, vitamins, and carotenoid yield on light energy in a flat-panel reactor using the A-stat technique. Biotechnol Bioeng, 89, 233-242.3.Ceron, M. C., Campos, I., Sanchez, J. F., Acien, F. G., Molina, E. and Fernandez-Sevilla, J. M. (2008) Recovery of Lutein from Microalgae Biomass: Development of a Process for Scenedesmus almeriensis Biomass. Journal of Agricultural and Food Chemistry, 56, 11761-11766.4.Gifuni, I., Pollio, A., Safi, C., Marzocchella, A. and Olivieri, G. (2019) Current Bottlenecks and Challenges of the Microalgal Biorefinery. Trends Biotechnol, 37, 242-252.5.Hu, J., Nagarajan, D., Zhang, Q., Chang, J.-S. and Lee, D.-J. (2018) Heterotrophic cultivation of microalgae for pigment production: A review. Biotechnology advances, 36, 54-67.6.Lin, J. H., Lee, D. J. and Chang, J. S. (2015) Lutein production from biomass: marigold flowers versus microalgae. Bioresour Technol, 184, 421-428.7.Ochoa Becerra, M., Mojica Contreras, L., Hsieh Lo, M., Mateos Diaz, J. and Castillo Herrera, G. (2020) Lutein as a functional food ingredient: Stability and bioavailability. Journal of Functional Foods, 66.8.Singh, D., Puri, M., Wilkens, S., Mathur, A. S., Tuli, D. K. and Barrow, C. J. (2013) Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters. Bioresour Technol, 143, 308-314.

4. 毕业设计(论文)计划

1-2月,安全培训,开题报告;

3-4月实验工作;

5月,撰写论文;6月答辩。

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