1. 毕业设计（论文）的内容和要求
论文内容从原绿球藻里把9215异构酶克隆出来， 然后连到PET-28a质粒上， 放在大肠杆菌里转录翻译，形成活性的蛋白。
论文要求1．文献查阅掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、Elsevier电子期刊、Springer Link全文电子期刊等检索资源，查阅关于表达纯化的方法以及N-乙酰葡萄糖异构酶性质的相关知识。
2．文献阅读及综述阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。
2. 参考文献
1. Baneyx F (1999) Recombinant protein expression in Escherichia coli. Curr OpinBiotechnol 10: 411421.2. Bowden GA, Paredes AM, Georgiou G (1991) Structure and morphology ofprotein inclusion bodies in Escherichia coli. Bio/Technology 9: 725730.3. Pan KL, Hsiao HC, Weng CL, Wu MS, Chou CP (2003) Roles of DegP inprevention of protein misfolding in the periplasm upon overexpression ofpenicillin acylase in Escherichia coli. J Bacteriol 185: 30203030. 4. Blix G., Gottschalk A. and Klenk E. (1957) Nature 179: 1088-10905. Blix G., Hoppe-Seylers Z. (1936) Physiol Chem 240: 43-546. Bulai T., Bratosin D., Pons A., Montreuil J. and Zanetta J. P. (2003) FEBS Lett534:185-1897. Crocker P. R., Clark E. A., Filbin M., Gordon S., Jones Y., Kehrl J. H. et al.(1998) Glycobiology 8(2): v-vi8. DeNinno M. P. (1991) Synthesis 8: 583-5939. Dreitlein W. B., Maratos J., Brocavich J. (2001) Clin Ther 23:327-35510. Endo T., Koizumi S., Tabata K., Ozaki A. (2000) Appl Microbiol Biotechnol 53:257-26111. Ferrero M. , Martnez-blanco H., Lopez-velasco. F., Ezquerro-senz. C., et al.(2007) Acta Biochim Pol 54: 387-39912. Funegard U. (1994) Eur J Cancer B Oral Oncol 308: 230-23313. Furuhata K. (2004) Trends Glycosci Glyc 16: 143-16914. Ghosh S., Roseman S. (1965) J Biochem 240: 1525-153015.Basu, A., Li, X., and Leong, S. S. (2011) Refolding of proteins from inclusionbodies: rational design and recipes, Appl Microbiol Biotechnol 92, 241-251.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
