代谢工程改造解脂耶氏酵母生产棕榈油酸任务书

 2021-10-22 09:10

1. 毕业设计(论文)的内容和要求

1.文献查阅掌握文献查阅的一般方法,学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。

2.文献阅读及综述阅读与课题相关的外文及中文文献,了解解脂耶氏酵母分子与发酵方面的文献,撰写文献综述。

3.明确实验任务,拟定实验方案根据所查阅文献的内容,确定实验内容及方案,拟定科学可行的研究计划。

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2. 参考文献

[1] 吴永美, 毛雪, 王书建, 等. 植物 ω-7 脂肪酸的系统代谢工程[J]. 植物学报, 2011, 46(5): 575-585.[2] Akazawa Y, Cazanave S, Mott J L, et al. Palmitoleate attenuates palmitate-induced Bim and PUMA up-regulation and hepatocyte lipoapoptosis[J]. Journal of hepatology, 2010, 52(4): 586-593.[3] Misra A, Singhal N, Khurana L. Obesity, the metabolic syndrome, and type 2 diabetes in developing countries: role of dietary fats and oils[J]. Journal of the American College of Nutrition, 2010, 29(sup3): 289S-301S.[4] Rybak A, Meier M A R. Acyclic diene metathesis with a monomer from renewable resources: Control of molecular weight and one‐step preparation of block copolymers[J]. ChemSusChem: Chemistry Wittmann, C., Advanced biotechnology: metabolically engineered cells for the biobased production of chemicals and fuels, materials, and health-care products. Angew Chem Int Ed 2015, 54, 3328-50.[7] Tai M, Stephanopoulos G. Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production[J]. Metabolic engineering, 2013, 15: 1-9.[8] Friedlander J, Tsakraklides V, Kamineni A, et al. Engineering of a high lipid producing Yarrowia lipolytica strain[J]. Biotechnology for biofuels, 2016, 9(1): 77.[9] Blazeck J, Hill A, Liu L, et al. Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production[J]. Nature communications, 2014, 5: 3131. [10] Liu H H, Madzak C, Sun M L, et al. Engineering Yarrowia lipolytica for arachidonic acid production through rapid assembly of metabolic pathway[J]. Biochemical engineering journal, 2017, 119: 52-58. [11] Markham K A, Palmer C M, Chwatko M, et al. Rewiring Yarrowia lipolytica toward triacetic acid lactone for materials generation[J]. Proceedings of the National Academy of Sciences, 2018, 115(9): 2096-2101.

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