1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解纳豆芽孢杆菌产维生素K2发酵过程，学习转录组学的分析方法和研究现状，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 参考文献
[1]．Walther B, Karl JP, Booth SL, et al. Menaquinones, bacteria, and the food supply: the relevance of dairy and fermented food products to vitamin K requirements [J]. Advances in Nutrition, 2013, 4(4): 463-473.[2]. Berenjian A, Mahanama R, Kavanagh J, et al. Vitamin K series: current status and future prospects [J]. Critical Reviews in Biotechnology, 2015, 35(2): 199-208.[3]. Koshihara Y, Hoshi K, Ishibashi H, et al. Vitamin K2 promotes 1 alpha,25(OH)(2) vitamin D-3-induced mineralization in human periosteal osteoblasts [J]. Calcified Tissue International, 1996, 59(6): 466-473. [4]. Hirao M, Hashimoto J, Ando W, et al. Response of serum carboxylated and undercarboxylated osteocalcin to alendronate monotherapy and combined therapy with vitamin K2 in postmenopausal women [J]. Journal of Bone and Mineral Metabolism, 2008, 26(3): 260-264. [5]. Shea MK, Holden RM. Vitamin K status and vascular calcification: evidence from observational and clinical studies [J]. Advances in Nutrition, 2012, 3(2): 158-165. [6]. 罗苗苗, 胡学超, 邱宏伟, 等. 微生物发酵法制备维生素K2研究进展 [J]. 食品与发酵工业, 2015, 42(10): 221-225.[7]. Suvarna K, Stevenson D, Meganathan R, et al. Menaquinone (vitamin K2) biosynthesis: Localization and characterization of the menA gene from Escherichia coli [J]. Journal of Bacteriology, 1998, 180(10): 2782-2787. [8]. Rowland B, Hill K, Miller P, et al. Structural organization of a Bacillus subtilis operon encoding menaquinone biosynthetic enzymes [J]. Gene, 1995, 167(1-2): 105-109. [9]. 李拖平, 郭梅, 王娜, 等. 枯草杆菌发酵生产VK2的技术工艺条件初探 [J]. 食品科学, 2008, 28(11): 379-382.[10]. Berenjian A, Mahanama R, Talbot A, et al. Efficient media for high menaquinone-7 production: response surface methodology approach [J]. New Biotechnology, 2011, 28(6): 665-672. [11]. Berenjian A, Chan NLC, Mahanama R, et al. Effect of biofilm formation by Bacillus subtilis natto on menaquinone-7 biosynthesis [J]. Molecular Biotechnology, 2013, 54(2): 371-378. [12]. Berenjian A, Mahanama R, Talbot A, et al. Designing of an intensification process for biosynthesis and recovery of menaquinone-7 [J]. Applied Biochemistry and Biotechnology, 2014, 172(3): 1347-1357. [13]. 张志强, 闵令华, 杨琰君, 等. 萘醌溶液的吸收和荧光光谱分析 [J]. 南京邮电大学学报(自然科学版), 2015, 33(01): 119-122.[14]. 向文胜, 王相晶, 任天瑞,等。
EPSP合成酶体外活性筛选模型特性 [C]. 成都：中国化工学会, 2002.111.[15]. Nusetti O, Tovar M, Zapatavvenes E. Pyruvate kinase, phosphoenolpyruvate carboxykinase, cytochrome c oxidase and catalase activities in cadmium exposed perna viridis subjected to anoxic and aerobic conditions [J]. Journal of Shellfish Research, 2013, 29(1): 203-208.[16]. Holms H. Flux analysis and control of the central metabolic pathways in Escherichia coli [J]. Fems Microbiology Reviews, 1996, 19(2): 85-116.[17]. Hu XC, Liu WM, Luo MM, et al. Enhancing menaquinone-7 production by Bacillus natto R127 through the nutritional factors and surfactant [J]. Applied Biochemistry and Biotechnology, 2017, DOI: 10.1007/s12010-017-2423-6.[18]. Fang ZW, Wang L, Zhao GH,et al. A simple and effiffifficient preparative procedure for menaquinone-7 from Bacillus subtilis (natto) using two-stage extraction followed by microporous resins[J]. Process Biochemistry, 2019, 83: 183-188.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
