合成1,5-二羟基萘的假单胞菌诱变和高通量筛选任务书

 2021-10-24 03:10

1. 毕业设计(论文)的内容和要求

1. 菌种诱变采用ARTP技术,对野生假单胞菌进行诱变,以期获得高产量菌株。

2. 高通量筛选建立有效的微生物高通量筛选方法,以期快速有效地筛选、分离出目标菌株。

要求:诱变和筛选试验,需反复进行,工作量较大,所以论文内容务必详实,通顺。

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2. 参考文献

Rodrigues, E. M., Kalks, K. H. M., Marcos, R. T. (2015). Prospect, isolation, and characterization of microorganisms for potential use in cases of oil bioremediation along the coast of Trindade. J Environ Manage.,156; 15-22. Seo, J. S., Keum, Y. S. and Li, Q. X. (2009). Bacterial degradation of aromatic compounds. Int J Environ Res Public Health., 6. Tsai, J., Kumar, M. and Lin, J. (2009). Anaerobic biotransformation of fluorene and phenanthrene by sulfate-reducing bacteria and identification of biotransformation pathway. J Hazard Mater., 164; 847-855. Varjani, S. J., and Upasani, V. N. (2016). Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514. Bioresour Technol., 222; 195-201. Waigi, M. G., Kang, F., Goikavi, C., Ling, W. and Gao, Y. (2015). Phenanthrene biodegradation by sphingomonads and its application in the contaminated soils and sediments: A review. Int Biodet Biodeg., 104; 333-349. Williams, E. S., Mahler, B. J. and Metre, P. C. V. (2013). Cancer Risk from Incidental Ingestion Exposures to PAHs Associated with Coal-Tar-Sealed Pavement. Environ Sci Technol., 47; 1101-1109. Zhang, G., Ling, J., Sun, H., Luo, J., Fan, Y. and Cui, Z. (2009). Isolation and characterization of a newly isolated polycyclic aromatic hydrocarbonsdegrading Janibacter anophelis strain JY11. J Hazard Mater., 172; 580-586. Zhou, L., Li, H., Zhang, Y., Han, S. and Xu, H. (2016). Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities. Braz J Microbiol., 47(2); 271-278.

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