全文总字数：3137字
1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解萜类化合物功能、解脂耶氏酵母合成萜类的途径及改造方法，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 实验内容和要求
具备条件：在Jens Nielsen教授的指导下，通过敲除解脂耶氏酵母的DNA 依赖的蛋白激酶亚基编码基因（ku70），有效的弱化了该酵母的非同源重组（NHEJ）能力，显著提升了其同源重组（HR）的能力，为后续在解脂耶氏酵母中异源导入木糖利用途径以及改造奠定了分子操作基础。
要求：在已经构建的敲除ku70解脂耶氏酵母工程菌株中异源表达木糖利用途径相关基因，同时通过实验室适应性策略，使解脂耶氏酵母能以木糖为唯一碳源生长。
3. 参考文献
Ledesma-Amaro, R., Z. Lazar, M. Rakicka, Z. Guo, F. Fouchard, A. C. Coq and J. M. Nicaud (2016). "Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose." Metabolic Engineering 38: 115-124.Li, H. and H. S. Alper (2016). "Enabling xylose utilization in Yarrowia lipolytica for lipid production." Biotechnology Journal 11(9): 1230-1240.Li, H. and H. S. Alper (2020). "Producing Biochemicals in Yarrowia lipolytica from Xylose through a Strain Mating Approach." Biotechnology Journal 15(2): e1900304.Nicaud, J. M. and R. Ledesma-Amaro (2018). "Engineering Yarrowia lipolytica to enhance lipid production from lignocellulosic materials." Biotechnology for Biofuels 11: 11.Yook, S. D., J. Kim, G. Gong, J. K. Ko, Y. Um, S. O. Han and S. M. Lee (2020). "High‐yield lipid production from lignocellulosic biomass using engineered xylose‐utilizing Yarrowia lipolytica." GCB Bioenergy.Ledesma-Amaro, R., Z. Lazar, M. Rakicka, Z. Guo, F. Fouchard, A. C. Coq and J. M. Nicaud (2016). "Metabolic engineering of Yarrowia lipolytica to produce chemicals and fuels from xylose." Metabolic Engineering 38: 115-124.Ong, K. L., C. Li, X. Li, Y. Zhang, J. Xu and C. S. K. Lin (2019). "Co-fermentation of glucose and xylose from sugarcane bagasse into succinic acid by Yarrowia lipolytica." Biochemical Engineering Journal 148: 108-115.Prabhu, A. A., R. Ledesma-Amaro, C. S. K. Lin, F. Coulon, V. K. Thakur and V. Kumar (2020). "Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control." Biotechnology for Biofuels 13: 113.Li, H. and H. S. Alper (2020). "Producing Biochemicals in Yarrowia lipolytica from Xylose through a Strain Mating Approach." Biotechnology Journal 15(2): e1900304.Wei, W., P. Zhang, Y. Shang, Y. Zhou and B. C. Ye (2020). "Metabolically engineering of Yarrowia lipolytica for the biosynthesis of naringenin from a mixture of glucose and xylose." Bioresource Technology 314: 123726.Wu, Y., S. Xu, X. Gao, M. Li, D. Li and W. Lu (2019). "Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica." Microbial Cell Factories 18(1): 83.Yao, F., S. C. Liu, D. N. Wang, Z. J. Liu, Q. Hua and L. J. Wei (2020). "Engineering oleaginous yeast Yarrowia lipolytica for enhanced limonene production from xylose and lignocellulosic Zhou, L., Z. Wen, Z. Wang, Y. Zhang, R. Ledesma-Amaro and M. Jin (2020). "Evolutionary Engineering Improved d-Glucose/Xylose Cofermentation of Yarrowia lipolytica." Industrial 毕业论文书写
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