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1. 毕业设计（论文）的内容和要求
1. 毕业论文内容1）建立多环芳烃羟基化反应样品处理方法；2）建立羟基化反应代谢产物分析方法。
2. 毕业论文要求1）建立有效、稳定的多环芳烃羟基化反应样品处理方法；2）建立准确性高的羟基化反应代谢产物分析方法。
2. 实验内容和要求
1. 比较2-3种文献报道的样品处理方法，分析样品组分回收率等；2. 比较3-5种文献报道的分析方法，优化分析过程。
3. 参考文献
Bugg, T. D. H. (2003). Dioxygenase enzymes: Catalytic mechanisms and chemicalmodels. Tetrahedron, 59(36), 70757101. https://doi.org/10.1016/S0040-4020(03)00944-X.Clark, L. C., Wolf, R., Granger, D., Taylor, Z. (1953). Continuous recording of bloodoxygen tensions by polarography. Journal of Applied Physiology, 18, 20. Retrieved fromwww.physiology.org/journal/jappl.Datsenko, K. A., Wanner, B. L. (2000). One-step inactivation of chromosomal genesin Escherichia coli K-12 using PCR products. Proceedings of the National Academy ofSciences of the United States of America, 97(12), 66406645. https://doi.org/10.1073/pnas.120163297.Davids, T., Schmidt, M., Bottcher, D., Bornscheuer, U. T. (2013). Strategies for the discoveryand engineering of enzymes for biocatalysis. Current Opinion in Chemical Biology,17(2), 215220. https://doi.org/10.1016/j.cbpa.2013.02.022.Ding, L., Tan, W., Zhang, Y., Shen, J., Zhang, Z. (2008). Sensitive HPLC-ESI-MSmethod for the determination of tiotropium in human plasma. Journal ofChromatographic Science, 46(5), 445449. https://doi.org/10.1093/chromsci/46.5.445.Ensley, B., Ratzkin, B., Osslund, T., Simon, M., Wackett, L., Gibson, D. (1983).Expression of naphthalene oxidation genes in Escherichia coli results in the biosynthesisof indigo. Science, 222(4620), 167169. https://doi.org/10.1126/science.6353574.Ferraro, D. J., Gakhar, L., Ramaswamy, S. (2005). Rieske business: Structure-function ofRieske non-heme oxygenases. Biochemical and Biophysical Research Communications,338(1), 175190. https://doi.org/10.1016/j.bbrc.2005.08.222.Fialkov, A. B., Steiner, U., Lehotay, S. J., Amirav, A. (2007). Sensitivity and noise inGC-MS: Achieving low limits of detection for difficult analytes. International Journal ofMass Spectrometry, 260(1), 3148. https://doi.org/10.1016/j.ijms.2006.07.002.Friemann, R., Lee, K., Brown, E. N., Gibson, D. T., Eklund, H., Ramaswamy, S. (2009).Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenaseenzyme system. Acta Crystallographica Section D: Biological Crystallography, 65(1), 2433.https://doi.org/10.1107/S0907444908036524.Gally, C., Nestl, B. M., Hauer, B. (2015). Engineering Rieske non-heme iron oxygenasesfor the asymmetric dihydroxylation of alkenes. Angewandte Chemie-International Edition,54(44), 1295212956. https://doi.org/10.1002/anie.201506527.Gibson, D. T., Koch, J. R., Kallio, R. E. (1968). Oxidative degradation of aromatic hydrocarbonsby microorganisms. I. Enzymatic formation of catechol from benzene.Biochemistry, 7(7), 26532662. https://doi.org/10.1021/bi00847a031.Gibson, D. T., Parales, R. E. (2000). Aromatic hydrocarbon dioxygenases in environmentalbiotechnology. Current Opinion in Biotechnology, 11(3), 236243. https://doi.org/10.1016/S0958-1669(00)00090-2.Guo, X. (Ed.), (2014). Advances in gas chromatography. IntechOpen.Guzman, L.-M., Belin, D., Carson, M., Beckwith, J. (1995). Tight regulation, modulation,and high-level expression by vectors containing the arabinose P(BAD) promoter.Journal of Bacteriology, 177(14), 41214130. https://doi.org/10.1128/jb.177.14.4121-4130.1995.Halder, J. M., Nestl, B. M., Hauer, B. (2018). Semirational engineering of the naphthalenedioxygenase from Pseudomonas sp. NCIB 9816-4 towards selective asymmetricdihydroxylation. ChemCatChem, 10(1), 178182. https://doi.org/10.1002/cctc.201701262.Hanahan, D. (1983). Studies on transformation of Escherichia coli with plasmids. Journal ofMolecular Biology, 166(4), 557580. https://doi.org/10.1016/S0022-2836(83)80284-8.Hentges, S. G., Sharpless, K. B. (1980). Asymmetric induction in the reaction of osmiumtetroxide with olefins. Journal of the American Chemical Society, 102(12), 42634265.
4. 毕业设计（论文）计划
2020.12-2021.1 文献综述2021.2-2021.3 样品处理2021.4-2021.5样品分析2021.5-2021.6 论文撰写、答辩
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