1. 毕业设计（论文）的内容和要求
1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解克雷伯氏菌分子与发酵方面的文献，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 参考文献
[1] Xu Y Z, Guo N N, Zheng Z M, et al. Metabolism in 1, 3‐propanediol fed‐batch fermentation by ad‐lactate deficient mutant of Klebsiella pneumoniae[J]. Biotechnology and Bioengineering, 2009, 104(5): 965-972.[2] Wang J, Zhao P, Li Y, et al. Engineering CRISPR interference system in Klebsiella pneumoniae for attenuating lactic acid synthesis[J]. Microbial cell factories, 2018, 17(1): 56.[3] Zhang Y, Li Y, Du C, et al. Inactivation of aldehyde dehydrogenase: a key factor for engineering 1, 3-propanediol production by Klebsiella pneumoniae[J]. Metabolic engineering, 2006, 8(6): 578-586.[4] Ko Y, Seol E, Sekar B S, et al. Metabolic engineering of Klebsiella pneumoniae J2B for co-production of 3-hydroxypropionic acid and 1, 3-propanediol from glycerol: reduction of acetate and other by-products[J]. Bioresource technology, 2017, 244: 1096-1103.[5] Lin H, Castro N M, Bennett G N, et al. Acetyl-CoA synthetase overexpression in Escherichia coli demonstrates more efficient acetate assimilation and lower acetate accumulation: a potential tool in metabolic engineering[J]. Applied microbiology and biotechnology, 2006, 71(6): 870-874.[6] Huang B, Yang H, Fang G, et al. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli[J]. Biotechnology and bioengineering, 2018, 115(4): 943-954.[7] Xu X, Xian M, Liu H. Efficient conversion of acetate into phloroglucinol by recombinant Escherichia coli[J]. RSC Advances, 2017, 7(80): 50942-50948.[8] Xiao Y, Ruan Z, Liu Z, et al. Engineering Escherichia coli to convert acetic acid to free fatty acids[J]. Biochemical engineering journal, 2013, 76: 60-69.[9] Noh M H, Lim H G, Woo S H, et al. Production of itaconic acid from acetate by engineering acid‐tolerant Escherichia coli W[J]. Biotechnology and bioengineering, 2018, 115(3): 729-738.[10] Li Y, Huang B, Wu H, et al. Production of succinate from acetate by metabolically engineered Escherichia coli[J]. ACS synthetic biology, 2016, 5(11): 1299-1307.[11] Peebo K, Valgepea K, Nahku R, et al. Coordinated activation of PTA-ACS and TCA cycles strongly reduces overflow metabolism of acetate in Escherichia coli[J]. Applied microbiology and biotechnology, 2014, 98(11): 5131-5143.[12] Yang J, Nie Q. Engineering Escherichia coli to convert acetic acid to β-caryophyllene[J]. Microbial cell factories, 2016, 15(1): 74.[13] Cheng K K, Liu H J, Liu D H. Multiple growth inhibition of Klebsiella pneumoniae in 1, 3-propanediol fermentation[J]. Biotechnology letters, 2005, 27(1): 19-22.[14] Jantama K, Polyiam P, Khunnonkwao P, et al. Efficient reduction of the formation of by-products and improvement of production yield of 2, 3-butanediol by a combined deletion of alcohol dehydrogenase, acetate kinase-phosphotransacetylase, and lactate dehydrogenase genes in metabolically engineered Klebsiella oxytoca in mineral salts medium[J]. Metabolic engineering, 2015, 30: 16-26.[15] Lin J, Zhang Y, Xu D, et al. Deletion of poxB, pta, and ackA improves 1, 3-propanediol production by Klebsiella pneumoniae[J]. Applied microbiology and biotechnology, 2016, 100(6): 2775-2784.
