1. 毕业设计（论文）的内容和要求
内容：1.MAPK相关基因的生物信息学分析2.构建MAPK的表达质粒3.重组质粒在酿酒酵母中的筛选4.胁迫下MAPK蛋白对于酿酒酵母生长状态的缓解情况5.渗透压胁迫下HSP蛋白对于酿酒酵母生长状态的缓解情况要求：熟悉实验室的基本操作安全规范使用实验仪器完成开题报告和毕业论文的写作1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。
2．文献阅读及综述阅读与课题相关的外文及中文文献，了解糙皮侧耳发酵产麦角硫因的方法及过程，撰写文献综述。
3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。
2. 参考文献
Jacob P, Hirt H, Bendahmane A. The heat‐shock protein/chaperone network and multiple stress resistance[J]. Plant biotechnology journal, 2017, 15(4): 405-414.McLoughlin F, Basha E, Fowler M E, et al. Class I and II small heat shock proteins together with HSP101 protect protein translation factors during heat stress[J]. Plant physiology, 2016, 172(2): 1221-1236.Zhao P, Guo Y, Zhang W, et al. Neurotoxicity induced by arsenic in Gallus Gallus: regulation of oxidative stress and heat shock protein response[J]. Chemosphere, 2017, 166: 238-245.Chen B, Feder M E, Kang L. Evolution of heat‐shock protein expression underlying adaptive responses to environmental stress[J]. Molecular ecology, 2018, 27(15): 3040-3054.Weidmann S, Maitre M, Laurent J, et al. Production of the small heat shock protein Lo18 from Oenococcus oeni in Lactococcus lactis improves its stress tolerance[J]. International journal of food microbiology, 2017, 247: 18-23.Ohama N, Sato H, Shinozaki K, et al. Transcriptional regulatory network of plant heat stress response[J]. Trends in plant science, 2017, 22(1): 53-65.Kampinga H H, Bergink S. Heat shock proteins as potential targets for protective strategies in neurodegeneration[J]. The Lancet Neurology, 2016, 15(7): 748-759.
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
