全文总字数：1429字
1. 毕业设计（论文）的内容和要求
1 文献阅读与归纳，深入了解课题背景2 构建邻苯二酚1,2-双加氧酶的突变株3 掌握重组酶酶学性质分析方法4 独立完成毕业论文的撰写
2. 实验内容和要求
1 掌握大肠杆菌重组菌的构建、诱导与表达的基本方法2 掌握酶活分析的基本操作3 掌握HPLC检测产物的方法
3. 参考文献
1 Long Y, Yang S, Xie Z, et al. Cloning, expression, and characterization of catechol 1, 2-dioxygenase from a phenol-degrading Candida tropicalis JH8 strain[J]. Preparative Biochemistry and Biotechnology, 2016, 46(7): 673-678.2 Han L, Liu P, Sun J, et al. Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli[J]. Scientific reports, 2015, 5: 13435.3 Xie N Z, Liang H, Huang R B, et al. Biotechnological production of muconic acid:current status and future prospects[J].Biotechnology advances,2014,32(3):615-622.4 Curran,K.A., Leavitt,J.M., Karim,A.S. , et al. Metabolic engineering of muconic acid production in Saccharomyces cerevisiae. Metabolic Engineering, 2013, 15:5566.
4. 毕业设计（论文）计划
2020.12-2021.01 阅读文献，完成开题报告2021.03-2021.04 突变株的构建2021.05-2021.05 重组酶酶学性质分析2021.06-2021.06 撰写毕业论文
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
