HPLC-FR检测血浆中伊立替康的活性代谢物SN-38的浓度任务书

 2021-10-15 21:06:54

1. 毕业设计(论文)的内容和要求

1、内容

在试验过程中,第一:制定出一套试验方案,也就是具体步骤,即:1、血浆的处理 2、分析物的提取3、提取物的挥干4、标准曲线的制定5、血浆样品的检测。

2、要求

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2. 参考文献

[1] Hu ZP, Yang XX, Chen X, Chan E, Duan W, Zhou SF. Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culturemedia and cancer cells by high performance liquid chromatography: application to cellular metabolism and accumulation studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2007. 850(1-2): 575-80.

[2] Zhang W, Dutschman GE, Li X, Ye M, Cheng YC. Quantitation of Irinotecan and its two major metabolites using a liquidchromatography-electrospray ionization tandem mass spectrometric. J Chromatogr B Analyt Technol Biomed Life Sci. 2009. 877(27): 3038-44.

[3] Bardin S, Guo W, Johnson JL, et al. Liquid chromatographic-tandem mass spectrometric assay for the simultaneousquantification of Camptosar and its metabolite SN-38 in mouse plasma and tissues. J Chromatogr A. 2005. 1073(1-2): 249-55.

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