疏水蛋白在大肠杆菌中的高效表达研究任务书

 2021-10-21 05:10

1. 毕业设计(论文)的内容和要求

1.文献查阅:掌握文献查阅,使用网络资源如中国期刊网、维普数据库、超星数字图书馆、Elsevier电子期刊、Springer Link全文电子期刊等检索资源,查阅关于脂肪酶产生菌产酶条件优化方面的相关文献。

2.文献阅读及综述:阅读与课题相关的外文与中文文献,了解国内外的研究动态,撰写文献综述。

3.明确实验任务,拟定实验方案:根据所查阅文献的内容,确定实验内容及方案,拟定科学可行的研究计划。

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2. 参考文献

[1] Kershaw M J, Talbot N J. Hydrophobins and Repellents: Proteins with Fundamental Roles in Fungal Morphogenesis[J]. Fungal Genetics Water Interface: Structural Analysis and Molecular Engineering哰J]. Biochemistry, 2007, 46(9):2345-2354.[12] Yamasaki R, Takatsuji Y, Lienemann M, et al. Electrochemical properties of honeycomb-like structured HFBI self-organized membranes on HOPG electrodes.[J]. Colloids Surfaces B Biointerfaces, 2014, 123:803-808.[13] Lo V C, Ren Q, Chi L L P, et al. Fungal Hydrophobin Proteins Produce Self-Assembling Protein Films with Diverse Structure and Chemical Stability[J]. Nanomaterials, 2014, 4(3):827-843.[14] Hakanp滗 J, Paananen A, Askolin S, et al. Atomic resolution structure of the HFBII hydrophobin, a self-assembling amphiphile.[J]. Journal of Biological Chemistry, 2004, 279(1):534-9.[15] Hakanp滗 J, Szilvay G R, Kaljunen H, et al. Two crystal structures of Trichoderma reesei hydrophobin HFBI桾he structure of a protein amphiphile with and without detergent interaction[J]. Protein Science, 2010, 15(9):2129-2140.[16] Hakanp悚盲 J, Linder M, Popov A, et al. Hydrophobin HFBII in detail: ultrahigh-resolution structure at 0.75 A[J]. Acta Crystallographica, 2006, 62(4):356?67.[17] Kershaw M J, Thornton C R, Wakley G E, et al. Four conserved intramolecular disulphide linkages are required for secretion and cell wall localization of a hydrophobin during fungal morphogenesis[J]. Molecular Microbiology, 2005, 56(1):117-25.[18] Kwan A H Y, Winefield R D, Sunde M, et al. Structural Basis for Rodlet Assembly in Fungal Hydrophobins[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(10):3621-3626.[19] Ren Q, Kwan A H, Sunde M. Solution structure and interface-driven self-assembly of NC2, a new member of the Class II hydrophobin proteins[J]. Proteins Structure Function Bioinformatics, 2014, 82(6):990-1003.[20] Chang H J, Lee M, Na S. Investigation of the role hydrophobin monomer loops using hybrid models via molecular dynamics simulation[J]. Colloids and Surfaces B: Biointerfaces, 2019, 173:128-138.

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