氨基化离子液体修饰的磁性壳聚糖—Cu2 螯合法固定化漆酶及其对有机污染物的降解研究任务书

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1. 毕业设计(论文)的内容和要求

1、毕业论文内容漆酶是一类以铜为催化活性中心的多酚类单电子氧化还原酶,具有较强的氧化还原能力且作用的底物相当广泛,包括许多芳香族化合物,如氯代酚、多氯联酚、芳胺、稠环芳烃、杀虫剂和染料等有机污染物。

但游离漆酶在使用过程中易受环境条件变化影响而变性失活,且不易从反应体系中分离重复使用,限制了其在有机废水治理领域的应用。

本课题拟发展一种新型的漆酶固定化方法,并将其应用于水中多种有机污染物的氧化降解。

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2. 参考文献

[1] Partha S. M., Gupta S. K. Effect of influent p H and alkalinity on the removal of chlorophenols in sequential anaerobic-aerobic reactors[J]. Bioresource Technology, 2009, 100(5): 1881-1883.[2] Mansoor A., Maryam L. Synthesis of amino-modified ordered mesoporous silica as a new nano sorbent for the removal of chlorophenols from aqueous media[J]. Chemical Engineering Journal, 2009,150(3): 555-560.[3] Hu J., Wang L. J. Degradation of chlorophenols in aqueous solution by γ-radiation[J]. Radiation Physics and Chemistry, 2007, 76(9): 1489-1492.[4] Zhou T., Li Y., Z., Wong F. S., et al. Enhanced degradation of 2,4-dichlorophenol by ultrasound in a new Fen-ton like system (Fe / EDTA) at ambient circumstance[J]. Ultrasonics Sonochemistry, 2008, 15(5): 782-790.[5] Lu Q. F., Yu J., Gao J. Z. Degradation of 2, 4-dichlorophenol by using glow discharge electrolysis[J]. Journal of Hazardous Materials, 2006, 136(3): 526-531.[6] Muriel A., Youssef F. M., Craig D. Removal estrogenic activity of natural and synthetic hormones from a municipal wastewater: Efficiency of horseradish peroxidases and laccase from Trametes versicolor[J]. Chemosphere, 2008, 70(3): 445-452.[7] Panneer S. S., Anil K., Rita K. An insilco approach to bioremediation: Laccase as a case study[J]. Journal of Molecular Graphics and Modelling, 2008, 26(5): 845-849.[8] Shigeyuki T., Chiak S., Kazuko S. Exoenzymes of Trametes versicolor can metabolize coplanar PCB congeners and hydroxy PCB[J]. Chemosphere, 2007, 67(9): 54-57.[9] Vianello F., Ragusa S., Cambria M. T., et al. A high sensitivity amperometric biosensor using laccase as biorecognition element[J]. Biosensors and Bioelectronics, 2006, 21(11): 2155-2160.[10] Khani Z., Jolivalt C., Cretin M., et al. Alginate/carbon composite beads for laccase and glucose oxidase encapsulation: Application in biofuel cell technology[J]. Biotechnology Letters, 2006, 28(22): 1779-1786.[11] Rogalski J., Dawidowicz A., Jozwik E. A., et al, Immobilization of laccase from cerrena unicolor on controlled porosity glass[J]. Journal of Molecular Catalysis B: Enzymatic, 1999, 6(1-2): 29-39.[12] Zhang J. B., Xua Z. Q., Chen H., et al, Removal of 2,4-dichlorophenol by chitosan-immobilized laccase from Coriolus versicolor[J]. Biochemical Engineering Journal, 2009, 45(1): 54-59.[13] 刘晓贞, 李 云, 梁云霄, 等, 二氧化硅/壳聚糖大孔复合材料固定化漆酶及其对2,4-二氯苯酚的降解[J]. 高校化学工程学报, 2016, 30(1): 201-209.

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