1. 毕业设计（论文）的内容和要求
内容：重组大肠杆菌的构建；酶的诱导表达；进一步优化利用木糖生产木糖醇的反应体系；要求：掌握微生物实验的基本规范操作，遵守实验室的安全规章制度。
2. 参考文献
1. Albuquerque, T. L.D.; da Silva, I. J.; de Macedo, G. R.; Rocha, M.V. P. Biotechnological production of xylitol from lignocellulosic wastes: A review. Process Biochem. 2014, 49, 17791789.2. Shah, S. S. M.; Luthfi, A. A. I.; Low, K. O.; Harun, S.; Manaf, S. F. A.; Illias, R. M;Jahim, J. M. Preparation of kenaf stem hemicellulosic hydrolysate and its fermentability in microbial production of xylitol by Escherichia coli BL21. Sci. Rep. 2019, 9, 1-13.3. Li, Z.; Guo, X.; Feng, X.; Li, C. An environment friendly and efficient process for xylitol bioconversion from enzymatic corncob hydrolysate by adapted Candida tropicalis. Chem. Eng. J. 2015, 263, 249-256.4. Su, B.; Wu, M.; Zhang, Z.; Lin, J.; Yang, L. Efficient production of xylitol from hemicellulosic hydrolysate using engineered. Escherichia coli. Metab. Eng. 2015, 31, 112-122.5. Murthy, G. S.; Sridhar, S.; Sunder, M. S.; Shankaraiah, B.; Ramakrishna, M. Concentration of xylose reaction liquor by nanofiltration for the production of xylitol sugar alcohol. Sep. Purif. Technol. 2005, 44, 221-228.6. Rafiqul, I. S. M.; Sakinah, A. M. M. Processes for the production of xylitol-a review. Food Rev. Int. 2013, 29, 127-156.7. Zheng, Y. G.; Yin, H. H.; Yu, D. F.; Chen, X.; Tang, X. L.; Zhang, X. J.; Xue, Y. P.; Wang, Y. J.; Liu, Z. Q. Recent advances in biotechnological applications of alcohol dehydrogenases. App. Microbiol. Biot. 2017, 101, 987-1001.8. Liu, Z. Q.; Lu, M. M.; Zhang, X. H.; Cheng, F.; Xu, J. M.; Xue, Y. P.; Jin, L. Q.; Wang, Y. S.; Zheng, Y. G. Significant improvement of the nitrilase activity by semi-rational protein engineering and its application in the production of iminodiacetic acid. Int. J. Biol. Macromol. 2018, 116, 563-571.9. Dasgupta, D.; Bandhu, S.; Adhikari, D. K.; Ghosh, D. Challenges and prospects of xylitol production with whole cell bio-catalysis: a review. Microbiol. Res. 2017, 197, 9-21.10. Mohamad, N. L.; Kamal, S. M. M.; Mokhtar, M. N. Xylitol biological production: a review of recent studies. Food Rev. Int. 2015, 31, 74-89.11. Pratter, S. M.; Eixelsberger, T.; Nidetzky, B. Systematic strain construction and process development: Xylitol production by Saccharomyces cerevisiae expressing Candida tenuis xylose reductase in wild-type or mutant form. Bioresour. Technol. 2015, 198, 732-738.12. Trichez, D.; Steindorff, A. S.; Soares, C. E. V. F.; Formighieri, E. F.; Almeida, J. R. M. Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production. FEMS Yeast Res. 2019, 19, foz034.13. Jang, S. H.; Kang, H. Y.; Kim, G. J.; Seo, J. H.; Ryu, Y. W. Complete In Vitro Conversion of n-Xylose to Xylitol by Coupling Xylose Reductase and Formate Dehydrogenase. J. Microbiol. Biotechn. 2003, 13, 501-508.
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