1. 毕业设计（论文）的内容和要求
1、采用计算机检索10篇中文文献和5篇英文文献；2、阅读15篇文献，翻译一篇英文文献，提交一篇开题报告。
格式要求为：参考文献标注符合规范，计算机打印。
3、实验工作主要内容分析骆驼刺泛菌NX-11的色素合成途径；使用CRISPR/Cas9对其关键基因进行无痕敲除；考察色素缺失后对胞外多糖产量的影响。
2. 参考文献
[1] Landrum, J. T. Bone, R. A. Lutein, zeaxanthin, and the macular pigment [J]. Archives of Biochemistry and Biophysics, 2001, 385(1): 28~40[2] Michael H. Walter Strack, D. Carotenoids and their cleavage products: biosynthesis and functions [J]. Natural Product Reports, 2011(28): 663[3] Preejith Vachali, Prakash Bhosale Bernstein, P. S. Microbial carotenoids [J]. Adv Biochem Eng Biotechnology, 1996(53): 119~178[4] Gregory A. Armstrong, Alberti, M. Hearst, J. E. Conserved enzymes mediate the early reactions of carotenoid biosynthesis in nonphotosynthetic and photosynthetic prokaryotes [J]. Proceedings of the National Academy of Sciences of the United States of America, 1990, 8(24): 9975~9979[5] Sandmann, G. Carotenoid biosynthesis and biotechnological application [J]. Archives of Biochemistry and Biophysics, 2001, 385(1): 4~12[6] Wu, X., Li, O. Chen, Y.et al. A carotenoid-free mutant strain of Sphingomonas paucimobilis ATCC31461 for the commercial production of gellan [J]. Carbohydrate Polymers, 2011, 84(3): 1201~1207[7] Zhu, L., Wu, X. Ou Liet al. Cloning and characterization of genes involved in nostoxanthin biosynthesis of Sphingomonas elodea ATCC31461 [J]. Plos One, 2012, 7(4): 35099[8] 朱亮. 少动鞘氨醇单胞菌产结冷胶优化及其念珠藻黄素合成相关基因的功能分析 [D].浙江大学，2013[9] NORIHIKO MISAWA, MASAYA NAKAGAWA KAZUO KOBAYASHIet al. Elucidation of the erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli [J]. Journal of Bacteriology, 1990, 172(12): 6704~6712
以上是毕业论文任务书，课题毕业论文、开题报告、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
