代谢工程提高解脂耶氏酵母油脂积累的研究任务书

 2021-11-04 08:11

1. 毕业设计(论文)的内容和要求

1.文献查阅掌握文献查阅的一般方法,学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、CA、SCI等外文数据库检索资源上查阅有关文献。

2.文献阅读及综述阅读与课题相关的外文及中文文献,了解解脂耶氏酵母分子与发酵方面的文献,撰写文献综述。

3.明确实验任务,拟定实验方案根据所查阅文献的内容,确定实验内容及方案,拟定科学可行的研究计划。

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2. 参考文献

[1] Kim, Minsuk, et al. "In silico identification of metabolic engineering strategies for improved lipid production in Yarrowia lipolytica by genome-scale metabolic modeling."Biotechnology for biofuels12.1 (2019): 187.[2] Teixeira, Paulo Gonalves, et al. "Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae."FEMS yeast research18.6 (2018): foy060.[3] Ferreira, Raphael, et al. "Metabolic engineering of Saccharomyces cerevisiae for overproduction of triacylglycerols."Metabolic Engineering Communications6 (2018): 22-27.Hapeta, Piotr, et al. "Transforming sugars into fatlipid biosynthesis using different sugars in Yarrowia lipolytica."Yeast34.7 (2017): 293-304.[4] Hapeta, Piotr, et al. "Transforming sugars into fatlipid biosynthesis using different sugars in Yarrowia lipolytica."Yeast34.7 (2017): 293-304.[5] Bhutada, Govindprasad, et al. "Sugar versus fat: elimination of glycogen storage improves lipid accumulation in Yarrowia lipolytica."FEMS yeast research17.3 (2017).[6] Qiao, Kangjian, et al. "Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica."Metabolic engineering29 (2015): 56-65.[7] Dulermo, Rmi, et al. "Role of Pex11p in lipid homeostasis in Yarrowia lipolytica."Eukaryotic cell14.5 (2015): 511-525.[8] Blazeck, John, et al. "Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production."Nature communications5 (2014): 3131.[9] Xue, Zhixiong, et al. "Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica."Nature biotechnology31.8 (2013): 734.[10] Dulermo, Thierry, et al. "Characterization of the two intracellular lipases of Y. lipolytica encoded by TGL3 and TGL4 genes: new insights into the role of intracellular lipases and lipid body organisation."Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids1831.9 (2013): 1486-1495.[11] Mihreteab, Merhawi, Bryan A. Stubblefield, and Eric S. Gilbert. "Microbial bioconversion of thermally depolymerized polypropylene by Yarrowia lipolytica for fatty acid production."Applied microbiology and biotechnology103.18 (2019): 7729-7740.[12] Gajdo, Peter, et al. "Overexpression of diacylglycerol acetyltransferase from Euonymus europaeus in Yarrowia lipolytica leads to the production of single‐cell oil enriched with 3‐acetyl‐1, 2‐diacylglycerols."Yeast(2019).[13] Leplat, Christophe, Jean-Marc Nicaud, and Tristan Rossignol. "Overexpression screen reveals transcription factors involved in lipid accumulation in Yarrowia lipolytica."FEMS yeast research18.5 (2018): foy037.[14] Pomraning, Kyle R., et al. "Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica."BMC genomics17.1 (2016): 138.[15] Morin, Nicolas, et al. "Transcriptomic analyses during the transition from biomass production to lipid accumulation in the oleaginous yeast Yarrowia lipolytica."PloS one6.11 (2011): e27966.

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