双重PCR方法检测大西洋三文鱼(Salmo salar)和虹鳟鱼(Oncorhynchus mykiss)任务书

 2021-10-24 03:10

1. 毕业设计(论文)的内容和要求

三文鱼是世界著名的高值经济鱼类之一, 其肉质鲜嫩, 鱼肉中富含ω-3不饱和脂肪酸, 营养丰富, 是深受中国老百姓喜爱的一种水产品。

在中国市场上, 三文鱼的主要品种是大西洋鲑鱼(Salmo salar) 和虹鳟鱼 (Oncorhynchus mykiss) 。

从分类学上讲, 大西洋鲑鱼和虹鳟都属于鲑形目鲑科鱼中的鲑亚科 (Salmoniae) 。

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2. 参考文献

[1] Xiong, X., et al., Multiple fish species identified from China's roasted Xue Yu fillet products using DNA and mini-DNA barcoding: Implications on human health and marine sustainability. Food Control, 2018. 88: p. 123-130.[2]. Shen, Y., et al., DNA barcoding for the identification of common economic aquatic products in Central China and its application for the supervision of the market trade. Food Control, 2016. 61: p. 79-91.[3]. Hebert, P.D.N., S. Ratnasingham and J.R. de Waard, Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences, 2003. 270(Suppl_1): p. S96-S99.[4]. Gnther, B., M.J. Raupach and T. Knebelsberger, Full-length and mini-length DNA barcoding for the identification of seafood commercially traded in Germany. Food Control, 2017. 73: p. 922-929..[5]. Cawthorn, D., H.A. Steinman and R.C. Witthuhn, Evaluation of the 16S and 12S rRNA genes as universal markers for the identification of commercial fish species in South Africa. Gene, 2012. 491(1): p. 40-48.

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